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商品详细U-CyTech/人IL-10 T细胞ELISPOT试剂盒/2-板/CT235-T2
U-CyTech/人IL-10 T细胞ELISPOT试剂盒/2-板/CT235-T2
U-CyTech/人IL-10 T细胞ELISPOT试剂盒/2-板/CT235-T2
商品编号: CT235-T2
品牌: ucytech
市场价: ¥6600.00
美元价: 3960.00
产地: 美国(厂家直采)
公司:
产品分类: ELISPOT试剂盒
公司分类: ELISPOT
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
IL-10 specific spots produced by 5x10E4 human PBMC.Stimulation: PMA/ionomycin.
Catalogue Number
CT235-T2
Description
Human IL-10 T cell ELISPOT kit - 2-plate
Price
€330.00
staining
Silver
Characteristics
Transparent
Contents
  • coating antibodies
  • biotinylated detector antibodies
  • phi-labeled goat anti-biotin antibodies
  • activator solutions
  • Blocking stock solution
  • Dilution buffer T
  • Tween-20
  • transparent ELISPOT plates with lids and cover slips
  • detailed manual

References list

  • Metabolic and immune effects of immunotherapy with proinsulin peptide in human new-onset type 1 diabetes.
    Alhadj Ali, Mohammad, et al. Sci Transl Med 2017 Aug 09;9:
  • Autoreactive T cell responses show proinflammatory polarization in diabetes but a regulatory phenotype in health.
    Arif, Sefina, et al. J. Clin. Invest. 2004 Feb;113: 451-63
  • Self-glycolipids modulate dendritic cells changing the cytokine profiles of committed autoreactive T cells.
    Buschard, Karsten, et al. PLoS ONE 2012;7: e52639
  • Development of type 1 diabetes despite severe hereditary B-cell deficiency.
    Martin, S, et al. N. Engl. J. Med. 2001 10 04;345: 1036-40
  • Development of novel LL-37 derived antimicrobial peptides with LPS and LTA neutralizing and antimicrobial activities for therapeutic application.
    Nell, Marja J, et al. Peptides 2006 Apr;27: 649-60
  • Beta-cell function in new-onset type 1 diabetes and immunomodulation with a heat-shock protein peptide (DiaPep277): a randomised, double-blind, phase II trial.
    Raz, I, et al. Lancet 2001 Nov 24;358: 1749-53
  • Islet antigen specific IL-10+ immune responses but not CD4+CD25+FoxP3+ cells at diagnosis predict glycemic control in type 1 diabetes.
    Sanda, Srinath, et al. Clin. Immunol. 2008 May;127: 138-43
  • CD8 T-cell reactivity to islet antigens is unique to type 1 while CD4 T-cell reactivity exists in both type 1 and type 2 diabetes.
    Sarikonda, Ghanashyam, et al. J. Autoimmun. 2014 May;50: 77-82
  • Comparison of cytokine ELISpot assay formats for the detection of islet antigen autoreactive T cells. Report of the third immunology of diabetes society T-cell workshop.
    Schloot, Nanette C, et al. J. Autoimmun. 2003 Dec;21: 365-76
  • Redirection of human autoreactive T-cells Upon interaction with dendritic cells modulated by TX527, an analog of 1,25 dihydroxyvitamin D(3).
    van Halteren, Astrid G S, et al. Diabetes 2002 Jul;51: 2119-25
  • Autoantibodies and associated T-cell responses to determinants within the 831-860 region of the autoantigen IA-2 in Type 1 diabetes.
    Weenink, S M, et al. J. Autoimmun. 2009 Sep;33: 147-54
  • Human mesenchymal stem cells modulate cellular immune response to islet antigen glutamic acid decarboxylase in type 1 diabetes.
    Zanone, Maria M, et al. J. Clin. Endocrinol. Metab. 2010 Aug;95: 3788-97
品牌介绍
U-Cytech位于荷兰,是全球著名的ELISPOT试剂盒生产商。生产各种人,大鼠,小鼠,猴,猩猩等ELISPOT试剂盒。产品丰富全面,质量一流。U-CyTech公司的透明ELISPOT培养板。该透明ELISPOT培养板的检测结果可以很方便的在倒置显微镜(普通的细胞室所具有的设备)下观察计数、或者采用酶联斑点自动图像分析仪分析。同时承载实验的培养板坚固耐用,容易清洗,不存在被加样枪头损坏的危险,使整个实验操作更为方便轻松。U-CyTech公司ELISPOT系统采用独特的黑色酶底物系统,显色系统非常灵敏并且稳定性更强,结果的保存期在6个月以上。U-CyTech公司提供的透明ELISPOT培养板预先进行了无菌处理。因此在包装未破损的情况下不需进行消毒处理。不过在细胞培养过程中还是应该注意无菌操作。红色或者蓝色斑点PVDF膜板经过干燥后可保存六个月。黑色斑点透明板干燥后可保存延长至两年。