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主营:主营:蛋白质分泌细胞的体外检测试剂
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商品详细U-CyTech/人IL-4 T细胞ELISPOT试剂盒/2-板/CT232-T2
U-CyTech/人IL-4 T细胞ELISPOT试剂盒/2-板/CT232-T2
U-CyTech/人IL-4 T细胞ELISPOT试剂盒/2-板/CT232-T2
商品编号: CT232-T2
品牌: ucytech
市场价: ¥6600.00
美元价: 3960.00
产地: 美国(厂家直采)
公司:
产品分类: ELISPOT试剂盒
公司分类: ELISPOT
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
IL-4 specific spots produced by 2x10E4 human PBMC.Stimulation: PMA/ionomycin.
staining
Silver
Characteristics
Transparent
Contents
  • coating antibodies
  • biotinylated detector antibodies
  • phi-labeled goat anti-biotin antibodies
  • activator solutions
  • Blocking stock solution
  • Dilution buffer T
  • Tween-20
  • transparent ELISPOT plates with lids and cover slips
  • detailed manual

References list

  • Autoreactive T cell responses show proinflammatory polarization in diabetes but a regulatory phenotype in health.
    Arif, Sefina, et al. J. Clin. Invest. 2004 Feb;113: 451-63
  • Immunological efficacy of heat shock protein 60 peptide DiaPep277 therapy in clinical type I diabetes.
    Huurman, V A L, et al. Clin. Exp. Immunol. 2008 Jun;152: 488-97
  • Development of type 1 diabetes despite severe hereditary B-cell deficiency.
    Martin, S, et al. N. Engl. J. Med. 2001 10 04;345: 1036-40
  • Beta-cell function in new-onset type 1 diabetes and immunomodulation with a heat-shock protein peptide (DiaPep277): a randomised, double-blind, phase II trial.
    Raz, I, et al. Lancet 2001 Nov 24;358: 1749-53
  • Comparison of cytokine ELISpot assay formats for the detection of islet antigen autoreactive T cells. Report of the third immunology of diabetes society T-cell workshop.
    Schloot, Nanette C, et al. J. Autoimmun. 2003 Dec;21: 365-76
  • Analysis of anthrax and plague biowarfare vaccine interactions with human monocyte-derived dendritic cells.
    Skowera, Anna, et al. J. Immunol. 2005 Dec 01;175: 7235-43
  • FTY720 treatment of kidney transplant patients: a differential effect on B cells, naïve T cells, memory T cells and NK cells.
    Vaessen, Leonard M B, et al. Transpl. Immunol. 2006 Apr;15: 281-8
  • Anti-CD25 therapy impairs donor-specific Th1 and Th2 cytokine-producing peripheral blood cells after clinical heart transplantation.
    van Besouw, N M, et al. Transplant. Proc. 2002 Nov;34: 2942-3
  • Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma.
    Vyth-Dreese, F A, et al. Clin. Exp. Immunol. 2010 Dec;162: 447-59
  • Human mesenchymal stem cells modulate cellular immune response to islet antigen glutamic acid decarboxylase in type 1 diabetes.
    Zanone, Maria M, et al. J. Clin. Endocrinol. Metab. 2010 Aug;95: 3788-97
品牌介绍
U-Cytech位于荷兰,是全球著名的ELISPOT试剂盒生产商。生产各种人,大鼠,小鼠,猴,猩猩等ELISPOT试剂盒。产品丰富全面,质量一流。U-CyTech公司的透明ELISPOT培养板。该透明ELISPOT培养板的检测结果可以很方便的在倒置显微镜(普通的细胞室所具有的设备)下观察计数、或者采用酶联斑点自动图像分析仪分析。同时承载实验的培养板坚固耐用,容易清洗,不存在被加样枪头损坏的危险,使整个实验操作更为方便轻松。U-CyTech公司ELISPOT系统采用独特的黑色酶底物系统,显色系统非常灵敏并且稳定性更强,结果的保存期在6个月以上。U-CyTech公司提供的透明ELISPOT培养板预先进行了无菌处理。因此在包装未破损的情况下不需进行消毒处理。不过在细胞培养过程中还是应该注意无菌操作。红色或者蓝色斑点PVDF膜板经过干燥后可保存六个月。黑色斑点透明板干燥后可保存延长至两年。