Description:

Plate characteristics:

Contents:

• Coating antibodies
• Biotinylated detector antibodies
• Phi-labeled goat anti-biotin antibodies
• Activator solutions
• Blocking stock solution
• Dilution buffer T
• Tween-20
• Transparent ELISPOT plates with lids and cover slips
• Detailed manual

References list:

• Immunological efficacy of heat shock protein 60 peptide DiaPep277 therapy in clinical type I diabetes. Huurman, V A L, et al. Clin. Exp. Immunol. 2008 Jun;152: 488-97
• Development of type 1 diabetes despite severe hereditary B-lymphocyte deficiency. Martin, S, et al. N. Engl. J. Med. 2001 Oct 4;345: 1036-40
• Association of T-cell reactivity with beta-cell function in recent onset type 1 diabetes patients. Pfleger, Christian, et al. J. Autoimmun. 2010 Mar;34: 127-35
• Comparison of cytokine ELISpot assay formats for the detection of islet antigen autoreactive T cells. Report of the third immunology of diabetes society T-cell workshop. Schloot, Nanette C, et al. J. Autoimmun. 2003 Dec;21: 365-76