U-CyTech/F(ab')2 fragments of mAb anti-human IFN-γ (clone MD-1)/CT215-F/1 Ea

来自 : 发布时间：2024-05-10

Description:

F(ab\')2 fragment antibodies are generated by pepsin digestion of whole IgG monoclonal antibodies to human IFN-γ (clone MD-1). F(ab\')2 fragments have two antigen-binding F(ab) portions linked together by disulfide bonds, while the Fc region is removed. F(ab\')2 fragments eliminate non-specific binding between Fc portions of antibodies and Fc receptors on cells (such as macrophages, dendritic cells, neutrophils, NK cells and B cells). 

MD-1 was first described in 1985. It has shown to neutralize the antiviral activity of both natural and recombinant human IFN-γ and in a Western Blot the antibody reacts with the 20 kDa and 25 kDa polypeptides present in natural human IFN-γ (van der Meide P.H. et al. 1985). The MD-1 antibody has been descriped as useful in a sandwich ELISA and in ELISPOT assays (Meyaard L. et al. 1996, Shen H. et al. 2013, Wassenaar A. et al. 1995 and Wierenga E.A. et al. 1990). Further, the antibody has proven to cross react with chimpanzee and rhesus macaque IFN-γ and can be used for immunohistochemical staining of frozen tissues (Jonker M. et al. 2013 and Kap Y. et al. 2009). The MD-1 antibody has been reported for use in intracellular flow cytometric analysis (Hamann D. et al. 1996) and in Luminex systems (Giavedoni L.D. et al. 2005).

Characteristics:

clone MD-1 [F(ab)2]